| dc.description.abstract | This project aimed to study phytochemical compositions, and biological activities of
Hedera Helix in Vietnam. Methanol extract of Hedera Helix were analyzed for chemical
compositions and bioactivities including antidiabetic and antibacterial. Triterpene
sapoponin had been identified from Hedera Helix leaf MeOH extract by LC-MS. Three
compounds including Hederacoside C, Hederasaponin B and Hederasaponin D were
successfully isolated from the Hedera Helix leaves by Medium pressure lipuid
chromatography and prep HPLC. Three compounds were structural elucidated by MS,
NMR in activity guided approach. The α-glucosidase inhibitory assay was used to test
antidiabetic activity by inhibiting the α-glucosidase enzyme, leading to reduce a
concentration of glucose absorbance. The result showed that the percentage of inhibition
of three compounds is not 50% sufficient to inhibit the α-glucosidase enzyme. The
antibacterial activity was also tested by the agar well diffusion method. The MIC results
of hederacoside C, Hederasaponin B and Hederasaponin are 4000ppm, 3000ppm,
3000ppm on Staphylococcus aureus and 3000ppm, 3000ppm, 3000ppm on Pseudomonas
aeuroginos, respectively. Hence, there are weak activities on three above tritepene
saponins. | en_US |
