| dc.description.abstract | Flinders Technology Associates (FTA) cards simplify sample storage, transport,
and extraction, therefore, the cost and time for diagnosis reduce. The purpose of
this research was to evaluate the efficacy of the Whatman® FTA® card in
inactivating bacterial cells and the storage of nucleic acids from bacterial cells that
infected aquatic animals. This study was designed with 2 experiments: the FTA
cards and filter papers were applied with different assumed bacterial
concentrations of Vibrio parahaemolyticus (101
, 103
, 106
, and 108 CFU/mL) and
infected Litopenaeus vannamei. After adsorption to FTA® Cards at room
temperature for 3 hours, V.parahaemolyticus was completely inactivated within
24h, 48h, and 72h. Bacteria from the FTA cards isolated on TCBS revealed that
there were no live bacterial cells from pure bacteria and infected shrimp whereas
the bacterial count for the filter papers increased significantly. The bacterial DNA
was sufficiently confirmed by the PCR technique, which was used to detect AHPND
disease focused on the gene of V. parahaemolyticus at different time conditions at
4ºC. Moreover, the bacterial nucleic acid was intact after storage for 3 months and
was PCR amplifiable. Overall, the results indicated that the FTA card was suitable
for the secure transport without contamination and storage of aquatic bacteria
under different conditions of a microbiological laboratory, as well as for the
detection of their nucleic acid over various storage times. | en_US |
