dc.description.abstract | Cashew nut testa (CSNT), the coat/cover of the cashew nut, is often discarded
because of its bitter, astringent taste though it concludes a quantity amount of
antioxidant compounds. Due to the above fact, the cashew nut testa extract (CSNTE)
would have lots of valuable hydrolyzable tannins that are vital to applied in the food
technology and essential to research.
In this study, to fabricate the use of CSNT, its extract loaded to the nanoparticles by
using double nanoemulsion method. Other components were Tween80 – hydrophilic
emulsifier, coconut oil – oil phase, double distilled water (DDW) – aqueous phase. In
two separated nanoemulsion system carried different emulsifiers, respectively,
Lecithin and Polyglycerol polyricinoleate (PGPR), and the ratios of CSNTE and carriers
was adjusted 5 – 10%. The physical properties (Z-Average, PDI, Zeta-potential) of
the CSNTE-loaded NPs, especially the Zeta-potential, were the indicator of stable
emulsion system to encapsulate bioactive compounds. Besides, its encapsulation
efficiency on functional activities, significantly delivered the percent of encapsulation
capacity of the NPs. The functional activities comprised of total phenolic content
(TPC), total flavonoid content (TFC), total catechin content (TCC).
The optimum ratios of CSNTE and carriers for both nanoemulsion system with Lecithin
and PGPR is 15:5. The primary nanoemulsion layer (W1/O) of the optimal CSNTE loaded NPs comprised of 15 wt% of CSNTE, 5 wt% of DDW in the internal water phase
(20 wt %); 5 wt% of hydrophobic emulsifier (Lecithin – experiment 01, PGPR –
experiment 02), 75 wt% of sesame oil in the oil phase (80 wt %). The final CSNTE loaded NPs by double nanoemulsion method (W1/O/W2) operated by 20 wt% of the
primary layer, 5 wt% hydrophilic emulsifier (Tween80) and 75 wt% of DDW. The EE
results of L15:5 was 69.542 ± 0.807 % of TCC, 73.774 ± 0.846 % of TFC, and 79.239
± 0.757 % of TPC. The EE results of P15:5 was 78.110 ± 0.983 % of TCC, 79.470 ±
0.613 % of TFC, and 83.176 ± 0.575 % of TCC.
This study provides the optimal ratios of CSNTE and carriers in formulating of stable
NPs, and the effects of lipophilic emulsifiers, Lecithin and Polyglycerol polyricinoleate
(PGPR) on the physical properties and encapsulation efficiency of NPs | en_US |