dc.description.abstract | Rhizoma curculiginis is a famous ethnopharmacological medicine for treatment of
various diseases, in general debility, deafness cough, asthma, piles, skin diseases,
impotence, jaundice, urinary disorders, leucorrhoea, menorrhagia, cancer and wound
healing and also the root is an official part and it contributes as a special drug in
treatment. The rhizomes was collected from Son La and Kon Tum are divided into
two parts: the water extract of whole rhizome serves for antibacterial testing while
the rest of them are separated into many small pieces of four types samples in steam
distillation (the bark and flesh of Son La, and the bark and the flesh of Kon Tum).
The four kinds of rhizomes were performed using steam distillation with 70%
methanol as a solvent, and the volatile organic compounds were identified by gas
chromatography - mass spectrometry (GC-MS). And, in many different conditions of
temperature (50°C, 55°C, 60°C, and 70°C) and durations (12 hours, 14 hours, 16
hours, and 24 hours) for the water extracts of rhizomes in two various sources Son
La and Kon Tum express the biological activities in antibacterial susceptibility
through agar disk diffusion method against Staphylococcus aureus (S. aureus) ATCC
6538, and Pseudomonas aeruginosa (P. aeruginosa) ATCC 27853. In this study, the
ethanol absolute was decided as the positive control, while distilled water was the
negative one. Besides, GC-MS is a modern tool to detect volatile organic compounds
and contribute a small amount of data for discovering little of very huge compounds
in natural herbs like the rhizome of Curculigo orchioides Gaertn, as well as
supporting the biological properties of its. Antimicrobial testing in many various
temperatures and duration to prove that the bioactivities of the extracts can resist
microorganisms, and find the optimal condition for collecting better data. | en_US |