| dc.description.abstract | Black pepper (Piper nigrum) is renowned as a popular spice used to flavor
dishes around the world and is also an agricultural export commodity that
brings high economic value to many countries. However, traditional
propagation methods by seed, cuttings, layering, and grafting reveal many
limitations such as the low multiplication coefficient, the low survival rate of
seedlings, and especially when there is an outbreak of disease, the ability to
control the disease source is exceedingly difficult, leading to the spread of
disease. Therefore, an alternative method through apical meristem
micropropagation has been applied to contribute to sustainable and disease free pepper cultivars. Propagation by apical shoot meristem gives high
propagation efficiency, good and uniform seedling quality, and can be
propagated in large numbers in a brief period. This study is conducted to
contribute to optimizing the process of micropropagation of pepper plants to
meet the needs of producing excellent quality seedlings, serving the
Vietnamese pepper industry. The two steps in the micropropagation sequence
studied in this project are shoot formation and bud cluster multiplication. In
this study, we investigate the effects of 6-benzylaminopurine (BA) and α Naphthalene acetic acid (NAA) in shoot formation. Regenerated shoots were
then cultured in MS media supplemented with different combinations of 6-
Furfurylaminopurine (Kinetin) and α-Naphthalene acetic acid (NAA) in bud
cluster multiplication experiment. The results showed that the apical shoot
meristem cultured on MS medium (Murashige and Skoog, 1962)
supplemented with 0.5 mg L-1 NAA and 2.0 mg L-1 BA was the optimal
medium for shoot formation rate (100%). Besides, MS culture medium
supplemented with Kinetin 1.5 mg L-1 + NAA 0.5 mg L-1 produced superior
results in bud cluster multiplication compared with other treatments (8, 20 ±
0.09 buds) after 8 weeks (about 2 months). | en_US |
