| dc.description.abstract | As a member of the Asfarviridae family, the African swine fever virus (ASFV) is a big,
double-stranded DNA virus. It is the agent responsible for African swine fever (ASF). African
swine fever is a viral disease of swine that has a high mortality rate in farmed pigs but is
asymptomatic in reservoir hosts. In the absence of a successful vaccine, the only known options
for disease control are quarantining the infected area and killing the affected animals. This method
of disease management might result in a substantial economic loss. The efficient and cost-effective
gathering of genetic data from ASFV field isolates is crucial for biosurveillance, limiting and
controlling its spread, and gaining a better understanding of the ecology of ASF illness. The current
Next-Generation Sequencing (NGS) method requires a large amount of host genome and viral
genome in an integrated collected sample in which the host genome is occupied largely by genetic
data (Hien et al., 2022). To avoid the unnecessary sequence of the host genes, the sequence
amplification of the viral genome followed by the NGS result reduces the cost and unexpected
time. In this study, long-range polymerase chain reactions (PCRs) are performed to amplify
portions of the ASFV genome. After the optimization, the developed method can amplify
approximately 45% of the ASFV genome, which is much improved from the previous result from
Meekins’s article with 37% of the coverage genome (Meekins et al., 2020). This project's end
result can help with research to find ways to stop ASF disease. | en_US |
