| dc.description.abstract | Cordyceps militaris (CM) has several bioactivities, including anticancer, anti inflammatory, antimicrobial, antioxidant, and immunomodulatory activity, thanks to
the presence of main bioactive compounds of cordycepin, adenosine, polysaccharide,
phenolic, and flavonoid. This study aimed to investigate the nutraceutical
compositions, including cordycepin, adenosine, carbohydrates, phenolic, and
flavonoid, and the biological activities such as antioxidant and antibacterial activity of
the C. militaris cultured in vitro in Vietnam provided by Vinseed Company. Cordycepin
and adenosine were quantified using High-Performance Liquid Chromatography
(HPLC). Along with, the quantification of total carbohydrates, phenolic, and flavonoid
content were assayed using phenol-sulfuric acid method, Folin-Ciocalteu assay, and
the aluminum chloride colorimetric assay, respectively. Subsequently, its antioxidant
activity was evaluated using DPPH assay, and its antibacterial property was
investigated using the agar well diffusion method, as well as minimum inhibition
concentration (MIC). The extraction yield of CM was 31.28% ± 0.03 obtained by
ethanol solvent from the ultrasonic-assisted extraction (UAE) method. In addition,
the cordycepin and adenosine content of the extract by High Performance Liquid
Chromatography (HPLC), was 4.096 mg/g (0.81%) and 0.935 mg/g (0.18%),
respectively. Furthermore, 10.70 mg/g, 20.87 ± 2.53 mg GAE/g, and 17.30 ± 4.56 mg
QE/g were the amounts of total carbohydrates, phenolics, and flavonoid, respectively.
Besides, the weak antioxidant activity of CM extract with IC50 = 1.25 mg/mL
compared to 14.72 μg/mL of ascorbic acid was recorded. Moreover, the ethanolic
extract of C. militaris exhibited antibacterial activity against Staphylococcus aureus
with a MIC value of 12.5 mg/mL, while the MIC value against Escherichia coli could
be not determined. Likewise, the antibacterial activity of CM extract tested by the
agar well diffusion method needs further study. In summary, C. militaris extract in
our study has comparable amount of bioactive compounds including cordycepins,
adenosine, polysaccharide, phenolic and flavonoid. It also has antioxidant and
antibacterial activity. This study provided useful data for further studies associated
with the investigation of the pharmacological activities of CM extract and the
development of functional foods and pharmaceutical products derived from CM. | en_US |
