| dc.description.abstract | Curcuma genus has been illustrating for possessing numerous medicinal treatments
such as antioxidant, anti-inflammation, hepatic problems, cough, diabetes rheumatism,
etc. and also willing to work as a skin lightening agent. C. longa L. is the most famous
in this genus for the ability to light up the skin which has been applied much in
cosmetic industry. The mechanism for this effect is the inhibitory ability on the
melanogenesis existing on our skin. Tyrosinase is an enzyme which participates
throughout the formation of melanin, a protein which can cause black spot on the skin.
Several inhibitors have been developed in order to prevent the activity of tyrosinase
leading to downgrade the melanogenesis. This project was performed to explore for a
new natural source of tyrosinase inhibitor from Curcuma genus by screening the
activity on 9 species from their total extracts with colorimetric quantitative assay on
96-well plate. The most potential extract would then be separated by preparative
chromatography (PC) method and screen again on the fractions. The active fractions
would then be analyzed via liquid chromatography – mass spectrometry (LC – MS) to
determine the active components. The results of this study demonstrated that C.
leucorrhiza had the potential to develop a new natural tyrosinase inhibitor with the
IC50 value was 47.97 ppm. The IC50 value of the active fractions was also calculated:
F2, IC50 = 98.31 ppm; F3, IC50 = 44.14 ppm; F4, IC50 = 98.71 ppm; F5, IC50 = 76.82
ppm; F6, IC50 = 94.29 ppm. Eleven compounds from these active fractions were
suggested by LC-MS and all belonged to currcuminoids. | en_US |
