| dc.description.abstract | Streptomyces spp., known for their prolific production of antimicrobial compounds,
provides a wealth of resources for potential therapeutic uses against various pathogenic
microbes. This study aimed to optimize the medium compositions (carbon and nitrogen
sources) and cultural conditions (pH) for enhancing antimicrobial production from
Streptomyces adustus, isolated in Con Dao Island, one of the promising strains for
antimicrobial activities. After screening five tested media at three distinct intervals,
tryptone soya broth (TSB) emerged as the most effective medium for antimicrobial
substance production at 144 hours. Ethyl acetate was used to extract antimicrobial agents
and for testing the extracts by the agar-well diffusion method. Subsequently, we
optimized this medium by varying the concentrations of dextrose, tryptone, and pH
within specified ranges (1-4g/L, 10-24g/L, and 5.0-9.0, respectively) using Box Behnken Design, in which the other ingredients were maintained at the original level.
We evaluated the effect of dextrose as a carbon source, tryptone as a nitrogen source,
and pH as a cultural condition through the 3D response surface plot. As a result, among
several tested conditions, the optimal range against Staphylococcus aureus (1.8 g/L
dextrose, 16.0 g/L tryptone, and pH 7.3) and Candida albicans (1.7 g/L dextrose, 17.5
g/L tryptone, and pH 8.0) was obtained. This study will be helpful in the implementation
of antimicrobial production on a large-scale fermentation. | en_US |
