A Study On Centella Asiatica Extract And Its Application In Serum Formulation

Abstract

The primary investigation of this study is the hydrating capacity of Centella Asiatica (C. asiatica) in serum formula. The C. asiatica was extracted by liquid pressure extraction and stored in semi-solid form for further bioactive analysis and cosmetic evaluation. The 2,2- diphenyl-1- picrylhydrazyl (DPPH) as radial scavenging assay, total phenolics content (TPC), total flavonoids content (TFC), liquid chromatography-mass spectrometry (LC-MS) and moist content were performed to identify the bioactive compound in the extract. Additionally, different concentrations of C. asiatica serum samples were used to compare cosmetic evaluation with base serum formula, which included sensory test, pH determination, spreadability test, penetration test, viscosity test, microbial growth assay, thermal stability test, hydrating capacity test. The extract inhibitor cytotoxic activity (IC50) value was reported to be 47.09 g/mL, compared with ascorbic acid, which was 14.86 g/mL. High potent antioxidant capacity correlation with high total phenolic and flavonoid content, reported respectively, 16.85  8.73 mg GAE/g and 630.6  8.73 mg QE/g. Four main bioactive triterpenes of C. asiatica were observed in LC-MS results, with several other bioactive compounds. The hydrating capacity of C. asiatica had a significant effect on the skin and increased with a concentration in serum formula. As a result, as shown from other cosmetic evaluations, the serum formula needs to be adjusted by adding another emulsifier to improve the stability of the product and sensory feeling. The hydrating capacity of C. asiatica was not significant enough to conclude its effect on the skin. Yet, the high potent antioxidant capacity and total phenolic and flavonoid contents in the extract might be used as supportive evidence for further research on the application of C. asiatica in cosmetic products.