Extraction Of Psidium Guajava ( Ruby Red) By Various Methods And Evaluation Of Antioxidant Activity Of Extracts

Abstract

Psidium guajava (type Ruby Red) was used in this study to extract antioxidants with the goal of providing the cosmetic industry. In the tropics, especially Vietnam, guava is a widely grown crop. Guava extract is predicted to offer an effective, affordable source of antioxidants for use in skin care products due to its low cost and claims that it contains high quantities of ascorbic acid (vitamin C) and antioxidant components. Ascorbic acid (vitamin C) and phytochemical compounds (including flavonoids and polyphenols) were extracted using Soxhlet extraction and ultrasound-assisted extraction with extraction durations of 15, 30, and 60 minutes at 45 degrees Celsius. The findings demonstrated that the use of ultrasound-assisted extraction for a brief period of time (15 minutes) did not produce the quite low results in any of the three assays for total phenolic content, total flavonoid content, and DPPH radical scavenging. Three different extraction times (15, 30, and 60 min) were tested utilizing ultrasound-assisted extraction on the three samples, and the findings tended to get better the longer the extraction time was. In both the DPPH radical scavenging assay (IC50 was 79.64 µg/mL) and the total phenolic content assay (180.05 ± 1.55 mg GAE/g extracts), Soxhlet extraction produced the best results. Additionally, the ultrasound-assisted extraction in 60 minutes produced the greatest results in the total flavonoid content testing, with 11.19 ± 0.24 mg QE/g extracts. While the Soxhlet extraction method requires more time and solvent than the other approach, the outcomes are only marginally better. In the total of three assays, the difference in results between ultrasound-assisted extraction in 30 and 60 minutes was not significant. Therefore, it is thought that ultrasound-assisted extraction in 30 and 60 minutes are the two most practical techniques