In Silico Discovery Microsatellite Markers In Gayal (Bos Frontalis)

Abstract

Gayal (Bos frontalis) is a hybrid of wild gaur and domestic cattle giving great value to agriculture in Vietnam along with the economics, which is now classified as endangered bovine species differing from domestic cattle such as Bos Taurus and Bos indicus. However, its genetic background remains unclear. Based on the database platform, we found the assembled genome is about 3.00 Gb with scaffold and contig N50 sizes of 1 Mb and 28.7 kb, respectively. In this research, in silico approach had been used to identify 350,119 microsatellite sequences found from the Gayal genome (Bos frontalis) by using MISA algorithm to search for different motifs of MSs. We then analyzed different statistics on those sequences with other species such as Bubalus Bubalis and perform primer design with in silico PCR to characterize the uniqueness of each STR. The result provides a useful resource for further genetic studies and finding polymorphism of Gayal on wild gaur. Deeper genetic studies to characterize the role of each STR in the conservation of this species are important for future research.