A Study On Glutathione Conjugated Sericin Nanoparticles In Cosmetics Applications

Abstract

The creation of cosmetic products that use Sericin nanoparticles to increase their efficacy and safety has drawn more attention in recent years. Silk Sericin nanoparticle Glutathione cosmetics, shown to have antioxidant properties, are promising. This study aimed to look into the conjugation formation of Glutathione-conjugated Sericin nanoparticles and their morphological characterization and antioxidant properties. Fourier transforms infrared (FTIR) analysis suggested the thiol functional group (2567.28 cm-1 ) of Glutathione in conjugation. Based on the difference in size and charge, Sericin nanoparticles and Glutathione-conjugated Sericin nanoparticles were analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS PAGE), which helped to support the hypothesis that Glutathione and Sericin nanoparticles are conjugated. Scanning electron microscopy (SEM) and Dynamic Light Scattering (DLS) tests were also used to check the morphological characterization of Glutathione-conjugated Sericin nanoparticles. The mean diameter of Glutathione-conjugated Sericin nanoparticles is 253 ± 11 nm, which is larger than the 224 ± 12 nm of Sericin nanoparticles. The zeta potential of Glutathione conjugated Sericin nanoparticles is -4.3 ± 0.23 mV. A colorimetric assay using the free radical scavenger picrylhydrazyl (DPPH) was also carried out. By using the DPPH Free Radical Scavenging Assay to measure antioxidant activity, Glutathione conjugated Sericin nanoparticles showed significant antioxidant capacity when compared to commercial Ascorbic acid, which had antioxidant capacities of 14.10 ± 0.039 and 15.36 ± 0.064 (µg/mL), respectively. Future studies are advised to confirm the stability of Glutathione-conjugated Sericin nanoparticles and their use in cosmetics. This study cannot be a procedure due to limitations in many areas.