Phytochemical Composition And Biological Activities Of Ilex Rotunda

Abstract

Chinese traditional medicine termed Ilex rotunda is used to treat acute gastroenteritis, diarrhea, tonsillitis, sore throats, and colds. Triterpenoid saponins are Ilex rotunda's primary bioactive components. The purpose of this research was to examine the chemical constituents and biological processes of this plant. A systematic strategy based on UPLC-Q-TOF-MS/MS was used for examining the mass spectra fragmentation and fracture modes in the anion mode using simple and efficient sample pretreatment and ultrasonic-assisted extraction processes. Following a comparison with the Dictionary of Natural Products database, thirteen chemical compositions were identified in total 554 compounds in Ilex rotunda. The large amount of bark of Ilex rotunda was extracted by ultrasound assisted machine. The total extract was obtained and liquid – liquid partition method conducted to remove some color pigments or impurities by n-hexane and ethyl acetate solvents. By utilizing solvent systems, the medium pressure liquid chromatography (MPLC) technique is employed to get fractions. Therefore, four condensed extracts, five fractions were obtained, and two compounds were effectively separated from isolation process. Analyzing the NMR data of Compound 1, it was determined as Pedunculoside- 3,19,23-Trihydroxy-12-ursen-28-oic acid; (3ß,19a)-form, 28-O-ß-D Glucopyranosyl ester, with molecular formula C36H58O10. After studying the NMR data, Compound 2 was identified as Syringin- 4-((1E)-3-hydroxyprop-1-en-1-yl)-2,6- dimethoxyphenyl beta-D-glucopyranoside, with molecular formula C17H24O9. There are extremely few research articles on Ilex rotunda's biological activities. By testing the activity on 11 samples using a colorimetric assay on a 96-well plate, this experiment was carried out to determine if Ilex rotunda exhibits biological activities in enzymes. As a result, α-amylase and lipase inhibition assays were carried out in this study. The lipase and amylase findings revealed that the materials (extracts, fractions, and compounds) exhibited no enzyme inhibitory effect.