Alkaloids Extraction And Aisolated Of Bioactive Compounds From Crinum Latifolium Leaves

Abstract

The aim of this project is to study about the extraction and isolation of alkloids and anticancer evaluation from Crinum latifolium in Vietnam. Three compounds including CL-CB0 (2.95g), CL-CA02 (0.0263g) and CL-CD0 (0.5149g) were isolated from the Crinum latifolium leaves by Medium pressure liquid chromatography (MPLC). Sulforhodamine B colorimetric assay (SRB assay) was performed to evaluate the inhibitory activity against MCF-7 cells growth. The total alkaloids extract exhibited higher cytotoxicity against MCF-7 cell line growth at screening concentration (percentage inhibition of 45.82±3.51 at 100 µg/mL) than three isolated compounds CL-CB0, CL-CA02 and CL-CD0 (percentage inhibition of 39.67±6.44%, 43.03±6.27% and 43.54±4.34% at 100µg/mL, respectively). Moreover, Crinamidine is an anticancer agent based on prevously reported studies. During this projects, crinamidine was also detected contains in Crinum latifolium leaves but it had not been completely isolated so it was not performed for testing anticancer. In conclusion, this study would serve as a foundation to the research in the future about the anticancer activity of the alkaloid compounds from Crinum latifolium and the application in medical and pharmaceutical industry.