Prevalence Of Viral Infections In Acute Respiratory Illness Patients At Hospitals In Ho Chi Minh City By Using Multiplex Real - Time PCR Assay

Abstract

Acute respiratory infections (ARIs) are a significant cause of death in developing countries such as Vietnam and are a worldwide challenge. Respiratory viruses also affect people of all ages, are highly infectious and spread from person to person. This study aims to evaluate the positive rate of viral infections and the distribution of 19 viral respiratory pathogens in acute respiratory illness patients at Ho Chi Minh City hospitals. Multiplex Real-time RT-PCR was used in this research to identify 19 common respiratory viruses in samples taken from 2051 ARI patients at hospitals in Ho Chi Minh City between July 2022 and January 2023. 1205 out of 2051 patients (58.75%) had virus-positive results. The virus-positive rates climbed from November 2022 (61.32%) to January 2023 (73.81%). Cytomegalovirus (CMV, 373/1205) and Epstein-Barr virus (EBV, 521/1205) were the viruses most frequently identified. The prevalence of CMV infections peaked in December and was relatively high in children (0–12 years) and senior adults (>=60 years). Infections with FluB increased in October 2022. Children were significantly more inclined to contract PIV-3, Rhinovirus, and Adenovirus illnesses, which peaked in December 2022 and January 2023. RSV infections peaked in July 2022, and Bocavirus infections peaked in July and December 2022; they were relatively more common in children. Infections with Human metapneumovirus peaked between October and December 2022. EBV infections peaked from September 2022 to January 2023, and SARS-CoV-2 infections peaked in November 2022; they were relatively more common in senior adults.Acute respiratory infections (ARIs) are a significant cause of death in developing countries such as Vietnam and are a worldwide challenge. Respiratory viruses also affect people of all ages, are highly infectious and spread from person to person. This study aims to evaluate the positive rate of viral infections and the distribution of 19 viral respiratory pathogens in acute respiratory illness patients at Ho Chi Minh City hospitals. Multiplex Real-time RT-PCR was used in this research to identify 19 common respiratory viruses in samples taken from 2051 ARI patients at hospitals in Ho Chi Minh City between July 2022 and January 2023. 1205 out of 2051 patients (58.75%) had virus-positive results. The virus-positive rates climbed from November 2022 (61.32%) to January 2023 (73.81%). Cytomegalovirus (CMV, 373/1205) and Epstein-Barr virus (EBV, 521/1205) were the viruses most frequently identified. The prevalence of CMV infections peaked in December and was relatively high in children (0–12 years) and senior adults (>=60 years). Infections with FluB increased in October 2022. Children were significantly more inclined to contract PIV-3, Rhinovirus, and Adenovirus illnesses, which peaked in December 2022 and January 2023. RSV infections peaked in July 2022, and Bocavirus infections peaked in July and December 2022; they were relatively more common in children. Infections with Human metapneumovirus peaked between October and December 2022. EBV infections peaked from September 2022 to January 2023, and SARS-CoV-2 infections peaked in November 2022; they were relatively more common in senior adults.