| dc.description.abstract | The ability of endophytic fungi to produce valuable bioactive compounds when surviving
in medicinal herbs. Finding out the biological sources is essential for pharmaceutical field.
Endophytic fungus was isolated from Angelica sinensis originated in Kon Tum province
(Vietnam). The isolation was based on the morphology of fungi. Identification was
performed by sequencing 18S rRNA that was compared with known genes using Blast
search in the combination of phylogenic analysis by using ClustalW and PhyML in
GenomeNet. The supernatant of isolated fungi was extracted and used to test antimicrobial
activitiy, which include antibacterial and antifungal activities. Antibacterial activitiy on
Staphylococcus aureus ATCC 6538, Pseudomonas aeruginosa ATCC 27853,
Micrococcus luteus ATCC 9341 and Klebsiella pneumoniae ATCC 10031 were carried
out by well diffusion method. In terms of antifungal activities on Aspergillus sp.,
Fusarium sp. were carried out by micro dilution method. As a result, Acremonium sp. F01
and Aspergillus sp. F02 were identified. All two isolated endophytic fungi extracts showed
biological activities. With antibacterial test, the extract of isolated fungi strain F01 in 150
μg/mL and 200 μg/mL concentration had the strongest inhibitory effect on K. pneumoniae
(13.37 ± 0.61 and 16.53 ± 0.72 mm) than S. aureus (10.33 ± 0.31 and 12.10 ± 0.56 mm),
M. luteus (8.13 ± 0.40 and 9.93 ± 0.50 mm) and P. aeruginosa (7.24 ± 0.31 and 9.70 ±
0.40mm). Especially, when the effect against K. pneumoniae was compared to
Ciprofloxacin 5 μg/mL, F01 with the concentration of 200 μg/mL gave inhibition zone
that was higher than positive control (16.53 ± 0.72 and 15.88 ± 0.61, respectively). With
antifungal test, the extract of isolated fungi strains F01 and F02 indicates high antifungal
activity against Fusarium sp. at 256 μg/mL concentration of MIC. With Aspergillus sp.,
the bioactive of F01 and F02 strain illustrated antifungal activity with MIC values of 16
μg/mL and 32 μg/mL, respectively. In conclusion, the study suggested fungus isolated
from Angelica sinensis could show and give many biological compounds that will be a
potential source for pharmaceutical field. | en_US |
