Preliminary Investigation For Phytochemical Compounds And Antioxidant Of Cordyceps Militaris From Vietnam

Abstract

Cordyceps Militaris has great potential as it can shed light on the common belief as traditional medicines which contains a variety of potential pharmaceutical properties. The present study aimed to compare phenolic compound and antioxidant of Cordyceps Militaris in three different solvents: Distilled water 100°C and 70°C, and ethanol 35°C. As for the method, 1 dried sample was extracted with 20 ml solvent (1:20) by using traditional method and speed extractor machine. The phenolic compound was investigated by The Folin-Ciocalteu method (TPC) and Quercetin (TFC). DPPH (1- diphenyl-2- picrylhydrazyl) was used to identify the antioxidant capacity. Cordycepin and adenosine content was determined by HPLC. As a result, the sample extracted with DW 70°C was the highest TPC and % DPPH inhibition was 12.9547 ± 0.4211 mg GAE /g and IC50 = 4.08 mg/g, while that with 100°C was the highest TFC, standing at 41.6476 ± 19.6898 mg QE /g. Sample extracted with 100°C was found that 2.04 mg/g cordycepin and 0.10 adenosine. Finally, Cordyceps were proposed as potential antioxidant properties by using distilled water extraction and speed extractor. However, due to some problems related to technique, source of materials, condition, and temperature, the results were not as good as expected.