| dc.description.abstract | Sericin, a protein extract from Bombyx Mori Silkworm, has been applied widely
in the cosmetics field. Sericin can be extracted through several methods, with or
without chemicals. In recent years, sericin has been developed into a new
biomaterial, by turning it into sericin nanoparticles. This study focused on
extracting sericin from silkworm cocoons with a high-pressure extraction method
and fabricating and characterizing the nanoparticles with a sericin solution.
Sericin extracted with the high-pressure method had a wide range of molecular
weights, it was suitable to apply two-step desolvation to fabricate. The
characterization was conducted with DLS, SEM, GPC, and FTIR together with the
Stability test. During the fabrication, the ratio between solvent: and nonsolvent
was investigated with 3 different ratios 1:2, 1:3, and 1:6 to observe the effect of
desolvating agent volume on the fabrication. In the study, it was observed that a
ratio of 1:2 resulted in high potential nanoparticles size, with 184±11.75 nm in
diameter. With the extremely negative charge, sericin nanoparticles from a ratio
of 1:2 was the most stable sample during 15 days of stability test. Sericin
nanoparticles had low molecular weight, with 20kDa particles making up the
majority in the population. In IR spectrum, there was a signal of conformational
change, which represented sericin had a slight change in secondary structure to
be sericin nanoparticle which was more stable . There was no transformation in
the functional group. Under the influence of a desolvating agent, the molecule
precipitated and created spherical stable nanoparticles. The most stable
nanoparticles were observed the morphology. On day 30, the morphology was
taken, some of them had disintegrated, and some of them still maintained their
spherical shape. The development and optimization of nanoparticle generation
from silkworm cocoons protein will contribute to the development of a new
biomaterial. Sericin nanoparticles are expected to be applied in many fields such
as medicine and the cosmetic industry. To be able to fully understand sericin
nanoparticles, a wide range of biochemical tests is highly recommended to be
able to comprehensively evaluate the applicability of sericin nanoparticles. | en_US |
