| dc.description.abstract | Sesame oil cake, derived as a by-product during the oil extraction process in the
industry, is commonly used as waste disposal, animal feed, or integrated into
composting practices. Nevertheless, in the modern day, sesame oil cake can be
recovered and utilized as a value-added product. The objectives of this study is to
determine the condition that gives the highest yield of total soluble polysaccharides
using enzyme-assisted extraction techniques with Viscozyme L. Four key factors were
considered: enzyme concentration (ranging from 0% to 4% v/w), sample/buffer ratio
(1:10, 1:20, 1:30, and 1:40 g/ml), incubation temperature (30°C, 40°C, 50°C, and
60°C), and incubation time (30, 50, 70, and 90 minutes). The impact of these factors
on polysaccharide yields was carefully examined. The results showed that the highest
polysaccharide yield was 28.36 %, obtained with the following conditions: pH 4.5, 2%
enzyme concentration, and a sample/buffer ratio of 1:30 g/ml at 50°C for 50 minutes.
Additionally, the study aimed to evaluate the physicochemical and functional
characteristics of the freeze-dried defatted sesame cake powder. The freeze-dried
polysaccharide powder revealed a moisture content of 4.31 ± 0.05%, ash content of
6.06 ± 0.09%, water solubility index (WSI) of 89.27 ± 0.64%, and a water holding
capacity (WHC) of 2.43 ± 0.18 gwater/gdry sample. The amount of total phenolic
content (TPC) of freeze-dried defatted sesame cake powder was estimated as Gallic
acid equivalents/100g spectrophotometrically following the Folin-Ciocalteus reagent
method, and the result was 861.23 ± mg GAE/100g. The radical scavenging activity
of sesame cake extracted powder was determined by DPPH assay, with an IC50 of
628.93 μg/ml. The overall results suggest that enzyme-assisted extraction represents a
highly effective approach, potentially valuable for the development of natural and safe
compounds in both the pharmaceutical and functional food industries. | en_US |
