| dc.description.abstract | This study was carried out to investigate the most suitable solvent mixture including types
and ratios for saponin extraction from Bao ginseng grown in Thanh Hoa. The simplex centroid design with four solvents as variable components including acetone, ethyl
acetate, methanol, ethanol was applied to optimize the extraction of saponin. The design
consisted of total 19 runs including pure solvents, binary mixtures, ternary mixtures, and
a quaternary mixture. Different Scheffé polynomial models including linear, quadratic,
and special cubic models were evaluated to find the most appropriate model that best fitted
the experimental data. Total saponin content was determined, and different solvent
systems had significant effects on the saponin extraction. The reduced quadratic model
was quite accurate, as shown by the high values of R2 (0.914), adjusted R2 (0.860), and
predicted R2 (0.664). The optimum conditions for the extraction of saponin from Bao
ginseng were the ternary mixture of acetone-ethyl acetate-methanol in the proportion of
0.36, 0.22, and 0.42, respectively. Determination of total phenolic content (TPC) and
antioxidant capacity (DPPH) was conducted after the optimum mixture conditions were
found. The ginsengs extract at optimum mixture conditions had 40.373 ± 0.045 mg
OAE/g db of total saponin content, 1.913 ± 0.041mg GAE/g db of total phenolic content,
and 4.640 ± 0.112 mg TE/g db of antioxidant capacity, indicating the confirmation of final
model. | en_US |
