| dc.description.abstract | There is a limited amount of preantral follicles inside the mammalian ovary, culture of
oocytes derived from preantral follicles (1-1.5 mm) is a promising technique as it takes
advantage of the enormous early-antral follicle source for culture and could be applied for
further research in Assisted Reproductive Technology (ART) like helping for ovarian cancer or infertile patient. Thus, Scientists have been working on culturing models of this
potential fertility reserve in ART to help infertile women but the low number of embryos
that develop from small eggs cultured in vitro presents a challenge for them. Therefore, a
novel culture model for small eggs from preantral follicles (1-1.5mm) is potential. In this
study, I investigated the effect of Astaxanthin (Ax) on development of Astaxanthin (Ax),
the most potent antioxidants found in nature on the development of meiotic competence
in early antral follicles (1-1.5 mm) following IVG and IVM. Oocytes- cumulus granulosa
complex (OCGC) were collected and cultured in an IVG medium supplemented with three
different concentrations of astaxanthin respectively,0μM, 0.25μM, and 0.5μM for 48
hours for IVG. The in vitro growth oocytes were subsequently cultured for 42 hours for
IVM to evaluate the capacity of the oocyte undergoing meiotic competence. The results
demonstrated that oocytes cultured in medium with 0.5μM and 0.25μM Ax x (81.00% and
79.33%, respectively) reached considerably the maturation stage than those cultured in
0μM Astaxanthin medium (39.33%). Moreover, to ensure the optimal quality of matured
oocytes, the expression of BMP15 and GDF9 was examined by Real-time qPCR.
Gathering all results, it is concluded that the addition of 0.5 μM Ax to IVG medium
improves the quality of porcine in vitro growth oocytes by exerting antioxidant impacts
on these oocytes. This novel culture medium can contribute to improving the in vitro
culture of human oocytes. | en_US |
