Molecular Identification And Mycelial Growth Characteristics Of Lyophyllum Strains From Pine Forest In Lam Dong Province

Abstract

Pine forest systems largely contribute to the natural ecology in general and to local community daily lives. One of the values that pine forests could bring to humans is the variety of mushrooms, the potential medicines as well as the nutritious food supplies. Numerous research on mushroom consumption has been conducted all over the world with 2,166 recorded edible species until 2004. Apart from commercialized mushrooms, there are multiple wild edible ones still not being investigated in Vietnam. In this study, we tried to apply molecular methods for identification of two mushrooms, coded with LyC and Ly37 strains, collected at three-needle pines forests in Da Nhim watershed protection forest. Owing to the multiple culinary, medicinal, and ecological potentials and the shortage of research on these strains in Vietnam, we decided to determine the genus and the best conditions of their mycelial growth to provide more evidence for further experiments. The results of sequencing and phylogeny showed that both obtained strains belong to clade Fumosum, section Difformia, Lyophyllum genus. After the successful genus determination, we proceeded to evaluate growth characteristics via dried mycelium biomass with different criteria such as cultured media, temperature, and pH values. The conclusion was made that although both LyC and Ly37 share some resemblance to the growth impact on Ohta media, 25oC and pH 5, they could be differentiated based on the growing ratio in some specific conditions. In the media and pH experiments, Ly37 had a tendency to favor malt and yeast extract also possess a broader pH adjustments than LyC while the mycelial development rate of this Ly37 strain was evaluated to grow more slowly than LyC in the temperature range from 15oC to 30oC.