dc.description.abstract | In this study, microwave- and ultrasound-assisted extraction with the aid of
enzyme treatment were carried out to find out the suitable conditions of combined
method when extracting polysaccharides and polyphenols from mangosteen peel.
Four factors: microwave power (80, 240, 400, 560W), irradiation time (4, 5, 6, 7
minutes), ultrasound temperature (40, 50, 60, 75˚C), ultrasound duration (20,
30, 40, 50 minutes) was employed to the effects on the polysaccharides yields
and polyphenols. For enzyme-microwave-assisted extraction, the highest
polysaccharides yield (17.37%) was obtained at 400W in 6 minutes, while the
highest total phenolic content value (68 mg GAE/g sample) was achieved at 400W
in 5 minutes. For enzyme-ultrasound-assisted extraction, the highest
polysaccharides yields and total phenolic content value under ideal condition
(50˚C in 40 minutes) were 18.86% and 79 mg GAE/g, respectively. Moreover, the
yields of polysaccharides and total phenolic content in combined methods
(enzyme-microwave-assisted extraction and enzyme-ultrasound-assisted
extraction) were significantly higher than those of enzyme treatment (control
sample) (p<0.05). Additionally, for both polysaccharides yields and total phenolic
content value, enzyme-ultrasound-assisted extraction was considered as a better
method to extract both polysaccharides and polyphenols from mangosteen peel.
Thus, applying enzyme-ultrasound-assisted extraction for extracting
polysaccharides and polyphenols from mangosteen peel can be utilized to reduce
agricultural wastes as well as provide economical value for bioactive compounds. | en_US |