| dc.description.abstract | Haddowia longipes, belonging to Ganodermataceae, is a potential medicinal mushroom thanks to its
bioactive components such as lanostanoid. However, no research has been conducted on its optimal
cultivation conditions and media compositions. Because the mycelial type (cottony or rhizomorphic;
the latter is more advantageous over the former, which results in much a higher fruiting body production
yield) and mycelial growth greatly influence the production of fruiting bodies, thus, optimizing the
factors affecting mycelial growth and type is the first step for the enhancement of mushroom cultivation
productivity. Therefore, this study was performed to investigate the effects of different media, pH
values, temperatures, nitrogen, and carbon sources (of the potential medium) on the formation of
mycelial type and growth of this fungal species.
A 5mm-diameter Cork borer was used to cut actively growing mycelium into equal blocks (each of
them served as an inoculum), then the inoculum was placed in the center of the appropriate nutrient
plate and incubated for 9 days in darkness. Four criteria including growth rate, dry weight, mycelial
density, and morphology were utilized for the evaluation of the effects. The obtained results found that
the optimal medium was Ohta medium (growth rate of 10.57 ± 0.43 mm/day, dry weight of 49.31 ±
3.56 mg); the optimal pH was 5.0 (growth rate of 11.12 ± 0.45 mm/day, dry weight of 27.7 ± 1.94 mg);
the optimal temperature was 30oC (growth rate of 11.67 ± 0.34 mm/day, dry weight 38.29 ± 5.34 mg).
The optimal nitrogen and carbon sources were peptone (growth rate of 11.85 ± 0.26 mm/day, dry weight
of 17.02 ± 1.76 mg) and mannitol (growth rate of 9.98 ± 0.36 mm/day, dry weight of 26.59 ± 1.79 mg).
Overall, the optimized medium and cultivation conditions for the best mycelial quality were Ohta
medium with a pH of 5 at 30oC, and peptone/ mannitol was the optimal nitrogen/ carbon source. This
medium and conditions resulted in a high growth rate and dry weight. | en_US |
