| dc.description.abstract | Although there is much evidence showing the applications of plasma laser in medical
fields such as healing wounds and eventually treating cancers, there are still limited
documents searching the relationship between plasma laser and embryos. Therefore, this
experiment was carried out in order to determine how plasma treatment affects the
preimplantation development of mouse embryos. This study was designed into a control
group and three small experimental groups which were not only compared with the
control group but also among them. In addition, plasma treatment was tested on the
embryos made from intracytoplasmic sperm injection (ICSI). After 6h hours of ICSI, plasma was radiated to the zygotes by three ranges corresponding with 2.50-2.60A
group, 2.80-2.89A group, and 2.90-3.00A group. As a result, the plasma-treated zygotes
could grow to the blastocyst stage. However, when the experimental group measured
with the control group, they showed a lower proportion (the highest percentage of the
blastocyst in the experimental group was 23.3% while the control took 36.7%). Moreover, the number of cells in the experimental group was less than the control (21,14, and 16 cell numbers respectively followed by 2.50-2.60A group, 2.80-2.89A group, and
2.90-3.00A group - different with 70 cell number in the control). Finally, the result
showed that plasma treatment has a negative effect on the preimplantation development
of mouse embryos because of reducing successful proportion. The implications of this
study may be used to promote further experiments in the future | en_US |
