| dc.description.abstract | This study aimed to investigate the expression of the green fluorescent protein (GFP)
gene during the preimplantation development of transgenic cloned bovine embryos.
Oocyte-cumulus-granulosa cell complexes (OCGs) were collected from bovine ovaries
for in-vitro maturation, and fibroblasts of Holstein cow were cultured for pMax-GFP
gene transfection and nuclear transfer. Somatic cell nuclear transfer was performed
to generate transgenic cloned bovine embryos by the advance of micromanipulation
system to collect the transfected cells. The expression of GFP proteins were observed
throughout transfected fibroblasts culturing and transgenic cloned bovine embryos
preimplantation development by using UV light. The results showed that the
expression of the GFP gene in fibroblasts decreased over time. At day 3, 42.3% of
the cells expressed the GFP gene, while at day 6, the percentage decreased to 15.6%.
By day 10, after cell passage, the percentage further decreased to 8.2%. Besides
that, the GFP gene was expressed in the 2-cell embryo and blastocyst stages of
preimplantation development of transgenic cloned bovine embryos. The GFP
expression was comparatively low during the 2-cell embryo stage. However, at
blastocyst stage the level of GFP expression were relatively high. The non-transfected
group had a higher percentage of embryos developing to the blastocyst stage at
29.6% compared to the transfected group at 21.05%. Additionally, the cell number
of the blastocyst was higher in the non-transfected group (102 cells) compared to the
transfected group (54 cells). These observations suggest that the foreign gene could
be expressed during the developmental progress of transgenic cloned bovine embryos
derived from transfected donor cells. The study highlights the potential utility genome
editing technology and reproductive biotechnology for generating transgenic cloned
bovine embryos, which could be particularly apply for further production of genetically
modified livestock with desirable traits. Overall, this study provides a better
understanding of the foreign gene expression in transfected cow fibroblast and during
early embryonic development that could contribute to the development of more
efficient methods for producing transgenic cloned production | en_US |
