| dc.description.abstract | Horseradish peroxidase (HRP) is widely used as a catalyst in biomedical
applications, but its natural enzyme properties present challenges. To address these
challenges, researchers have studied an artificial alternative: magnetic nanoparticles
coated with gelatin-hematin (MNPs-Ge-He), which mimic HRP function with
improved characteristics. Hematin is a traditional substitute for HRP due to its
similar heme group, but its poor solubility in neutral media hinders its use in medical
applications. To overcome this, hematin was bound with gelatin as a conjugate. The
HRP-mimicking catalyst (MNPs-Ge-He) was synthesized by coating magnetic iron
oxide nanoparticles (MNPs) with (3-trimethoxysilyl) propyl-ethylenediamine
(TSPED) and functionalizing them with gelatin-hematin (Ge-He). Fourier transform
infrared spectroscopy (FTIR) confirmed that the MNPs-Ge-He synthesis was
effective, and X-ray diffraction (XRD) assessment showed that TSPED, gelatin, and
hematin did not affect the crystal structure of the MNPs. Synthetic catalyst
magnetisms demonstrated a magnetic function in catalyst recovery in VSM patterns.
DLS and TEM analyses confirmed the successful fabrication of MNPs-Ge-He. The
enzymatic oxidation of the catalyst was determined using rutin and ABTS assays.
MNPs-Ge-He was applied in resveratrol polymerization as a mimicking HRP catalyst. The polymerization process was carried out for different lengths of time
(one, two, three, and six hours), and the most effective reaction time was determined
using DPPH antioxidant assay. Overall, the study showed that MNPs-Ge-He is an
effective alternative to HRP with improved properties, paving the way for its use in
various biomedical applications. | en_US |
