| dc.description.abstract | The ‘optimal’ conditions of the in vitro growth (IVG) process in pre-antral follicles of
porcine, since it shared similarities with human is very necessary to increase the supply of
grown and matured oocytes for other research. Moreover, as an antioxidant, Ascorbic Acid
has shown its function of reducing ROS in culturing of maturation oocytes, but not for pre antral follicles. 2D system in the IVG process of porcine oocytes derived from pre-antral
follicles also not have been researched successfully. Thus, this research’s purpose is to
examine the role of Ascorbic Acid in in vitro growth of porcine oocytes derived from the pre antral follicles, together evaluate the efficiency of IVG culture of pre-antral follicles among
2D system on normal plastic dish and on extracellular matrix. First, OGCs from pre-antral
follicles were culture on IVG for 7-10 days with supplemented of AA (0µM, 50µM, 100µM),
then the effect of AA on morphology, Chromatin configuration and Histone modification was
examined. Afterward, the ‘optimal’ concentration of AA was used in examined the effect of
Laminin and Agarose coated dish for IVG oocyte derived from pre-antral follicles. Based on
the final result, AA at a concentration of 50µM maintained the higher quality of oocyte, better
cumulus granulosa cells’ proliferation, higher proportion of oocyte survived (71.15%), antral
forming (24.77% on Day 3 and 32.18% on Day 7), and higher of GV presence (13%) after
the IVG process, compared to 0µM and 100µM. Furthermore, 2D culture system using
Laminin and Agarose also presented improved in morphology, survival and antrum
formation, duration (up to Day 14 for Agarose coated dish). To sum up, during IVG culture of
porcine oocytes derived from pre-antral follicles, AA at a concentration of 50µM and 2D
systems available of Laminin and Agarose demonstrate the optimal condition for culturing
the OGCs, as it improved morphology, survival, antrum formation and chromosome
configuration of oocytes. | en_US |
