Effects Of Recipient Oocytes And Donor Cells On The Preimplantation Development Of Interspecies Cloned Embryos Between Porcine And Bovine

Abstract

This study was carried out to examine the effect of recipient oocytes and donor cells on the preimplantation development of interspecies cloned embryos between porcine and bovine. The study investigated the in vitro development, and the timing of embryo division during the first mitotic cell cycle and during preimplantation development. Porcine and Bovine intraspecies SCNT embryos were used as controls for comparison. The examination of the data indicated that foreign oocytes could reprogram somatic cells from distinct species and foster their growth to the blastocyst phase in vitro. Nonetheless, the cleavage rates of interspecies SCNT embryos (24.1%) were markedly lower than those of intraspecies SCNT embryos with 51.7% in Porcine Porcine group and 80.0% in Bovine-Bovine group. The rate of blastocyst formation was not significantly different among the three groups, with 21.4% for Porcine-Bovine SCNT embryos, 53.3% for Porcine-Porcine SCNT embryos, and 37.5% for Bovine Bovine SCNT embryos. The number of cells per blastocyst in the Porcine-Bovine group was similar to that of the Porcine-Porcine group (64 ± 7.2 and 49.3 ± 10.2, respectively), but it was significantly lower than the number of cells per blastocyst in the Bovine-Bovine group (115.3 ± 4.6) (P<0.05). Furthermore, there was no difference on the timing of the first mitotic cell cycle among interspecies and intraspecies cloned embryos. During the examination of the timing of embryo division in the preimplantation development, interspecies cloned embryos displayed a noticeable distinction in the timing of embryo division compared to intraspecies cloned embryos, from the 2-cell stage to the 8-cell stage. Subsequently, there was no significant difference in the timing of blastocyst formation among the three groups. In conclusion, this study indicated that the preimplantation development of interspecies cloned embryos was influenced by the recipient oocytes. In the future, this may provide insight into the factors that influence the success of interspecies cloning and enhance our understanding of the molecular and cellular mechanisms during preimplantation development in interspecies cloned embryos.