Study on drug delivery for paclitaxel of the lactobacillus strains

Abstract

The practice of developing molecularly targeted drugs to achieve a higher degree

of cancer therapy and antibiotic resistance is indispensable. Lactobacillus strains participated in the anti-cancer effects and performed the high-level specificity for cancer cell lines. This paper discussed the ability of minicells which were generated from Lactobacillus acidophilus VTCC-B-871 and Lactobacillus rhamnosus JCM 15113 for drug delivery of paclitaxel. L. acidophilus VTCC-B-871 and L. rhamnosus JCM 15113 formed minicells with highly significant number (1,070,000 and 787,000 minicells, respectively) in modified MRS broth with the concentration of fructose 10 g/l and lactose 50 g/l, respectively. Nanoparticle size of obtained minicells ( 400 nm in diameter) was determined using scanning electron microscopy. The minicells packaged paclitaxel (10 µg/ml) and cephalosporin (10 µg/ml) for different times of incubation (10, 15 and 24 hours) at 37°C. Our results showed that drugs could be completely absorbed for 10 hours by detecting extracted solution from drug packaged minicells on antimicrobial activities. The significant influence of different concentrations of loading paclitaxel (5, 10, 20 µg/ml) on drug packaging was studied. The results indicated that after extraction of the paclitaxel packaged minicells and analysis with high performance liquid chromatography for determining the number of paclitaxel presented in a minicell, there was a huge amount of paclitaxel encapsulated in a minicell (31 million paclitaxel molecules per minicell with loading paclitaxel (20 µg/ml)). The present work was the first report on the generation of minicells from Lactobacillus acidophilus VTCC-B-871 and Lactobacillus rhamnosus JCM 15113 and the drug delivery for paclitaxel of these minicells.

Key works: Lactobacillus acidophilus, Lactobacillus rhamnosus, minicells, paclitaxel, cephalosporin, antimicrobial activities, high performance liquid chromatography.