Investigation Of Wound Healing Effect Of Electrospun Membrane Containing Nano Curcumin And Tamanu Oil On Mouse Model

Abstract

Wound healing presents complex biomedical challenges, particularly in the context of chronic wounds and impaired healing mechanisms. This study explores the potential of electrospun membranes composed of polycaprolactone (PCL) and poloxamer 407 (POX), enriched with nano curcumin and tamanu oil, as advanced wound care solutions. Through a comprehensive research framework, including fabrication, characterization of morphology and density of coated solution onto the fabricated membranes, characteristics of the prepared membranes were demonstrated and evaluated through standard experimental methods. In vitro antibacterial assay, in vivo evaluations of mouse model and gene expression analysis indicated the efficacy of these membranes. Results revealed that higher concentrations of tamanu oil contributed to enhanced coating density and improved wound healing efficacy. Controlled release kinetics of curcumin and tamanu oil from the membranes were demonstrated, along with significant antibacterial properties against Staphylococcus aureus and Pseudomonas aeruginosa. In vivo studies on a mouse model demonstrated accelerated wound closure, tissue regeneration, and inflammation reduction, particularly with membranes containing higher concentrations of tamanu oil. Histological and gene expression analyses provided mechanistic insights into the enhanced wound healing process. Overall, this study highlights the potential of electrospun PCL/POX membranes coated with nano curcumin and tamanu oil as promising candidates for advanced wound care strategies, with implications for the development of effective therapies for chronic and non-healing wounds.