Calophyllum Inophyllum Oil - Encapsulated Pcl/Pox 407 Electrospun Membrane For Wound Dressing Applications: From Surface Topographical Analysis To Antibacterial Activity

Abstract

This thesis primarily delineates the fabrication process of electrospun membranes comprising PCL/POX/CIO for antibacterial wound dressings. Within the scope of this thesis, the membranes underwent comprehensive characterization through various tests and experiments. The principal objective of this study pertains to the discernment of the supremely efficacious concentration among the triad of constituents, namely PCL, POX, and CIO. During the preliminary phase of the characterization stage, the topographical surface morphology was conducted utilizing SEM. This allowed for the observation of the fiber matrices and the formations of beads. Consequently, it can be concluded that there is a proportional correlation between the CIO concentration and the fiber diameter. Furthermore, as the concentration of POX increased, beads were discovered. Theoretically, as water absorption capacity is one of many primary criteria for an effective wound dressing product, contact angle and water absorbability test were therefore performed. The results have strongly emphasized on the role of POX, which significantly enhances the water absorption capacity as its concentration increased. Despite its undeniable role, POX was discovered to significantly reduce the mechanical strength of the electrospun membrane through the tensile test due to bead formations. Furthermore, FTIR was employed to ascertain the presence of chemical functional groups about the constituent substances. In order to confirm moisture permeability, MVTR was also performed. Lastly, obtained membranes were investigated for their antibacterial activity with S.aureus and P.aeruginosa using the agar diffusion technique. As a result, membranes exhibited good antibacterial properties in S.aureus, proving that this PCL/POX/CIO membrane has promising potential in the future.