Research On The Application Of Low-Level Laser Therapy Using Red-Light Wavelength 655 Nm In Hek293 Cell Line Proliferation

Abstract

The HEK293 cell line, originating from human embryonic kidney cells, is important in biomedical engineering because of its simple cultivation and effectiveness in manufacturing recombinant proteins. This research explores how Low-Level Laser Therapy (LLLT) can enhance cellular research and treatments by studying its application on HEK293 cells. A LLLT device, made with a copper board that was soldered, was utilized to expose HEK293 cells to power intensities of 7 mW/cm² and 14 mW/cm² for different time periods. The impact of irradiation was evaluated by measuring cell proliferation using growth patterns and absorbance readings at 570 nm and 630 nm. Findings showed that LLLT notably enhanced cell proliferation, with best results seen at particular irradiation levels 7 mW/cm² for 3 minutes and 20 seconds and 14 mW/cm² for either 1 minute and 5 seconds or 2 minutes and 10 seconds. Despite being effective, the device has been found to have drawbacks like inconsistent light distribution and inaccuracies in measurement. Anticipated enhancements in LED control and design refinement are expected to improve the device's performance in the future. This research showcases the potential of LLLT in biomedical research and therapeutic uses, opening doors for future advancements in its implementation and precision.