| dc.description.abstract | The HEK293 cell line, originating from human embryonic kidney cells, is important in
biomedical engineering because of its simple cultivation and effectiveness in
manufacturing recombinant proteins. This research explores how Low-Level Laser
Therapy (LLLT) can enhance cellular research and treatments by studying its
application on HEK293 cells. A LLLT device, made with a copper board that was
soldered, was utilized to expose HEK293 cells to power intensities of 7 mW/cm² and
14 mW/cm² for different time periods. The impact of irradiation was evaluated by
measuring cell proliferation using growth patterns and absorbance readings at 570 nm
and 630 nm. Findings showed that LLLT notably enhanced cell proliferation, with best
results seen at particular irradiation levels 7 mW/cm² for 3 minutes and 20 seconds and
14 mW/cm² for either 1 minute and 5 seconds or 2 minutes and 10 seconds. Despite
being effective, the device has been found to have drawbacks like inconsistent light
distribution and inaccuracies in measurement. Anticipated enhancements in LED
control and design refinement are expected to improve the device's performance in the
future. This research showcases the potential of LLLT in biomedical research and
therapeutic uses, opening doors for future advancements in its implementation and
precision. | en_US |
