Drug Delivery Materials Based In Modified Chitosan

Abstract

In this study, we undertook the synthesis of a copolymer of Chitosan and Brij S100 (CTS-Brij S100) for encapsulating the antibiotic Tetracycline. Following each synthetic step, the products were characterized using FT-IR, 1H- NMR, and CMC analyses. The size of Tetracycline-loaded nanoparticles was determined by Dynamic Light Scattering (DLS) to be 85.5 ± 1.913 nm. The existence of fundamental bonds between CTS and cross-linking agents in the formation of nanoparticles was evidenced through FT-IR spectroscopy. The efficiency of Tetracycline encapsulation in the nanosystem was assessed by UV-Vis spectrophotometry, revealing that the nanosystem could release Tetracycline slowly in two different pH environments, 7.4 and 3.0, with release rates of 67.5099% and 70.9047% respectively over 48 hours. The CTS-Brij S100 system demonstrated the ability to inhibit the growth of Methicillin-resistant Staphylococcus aureus (MSSA ATCC 6538) through the measurement of bacterial inhibition zone diameters and the minimum inhibitory concentration (MIC) at various concentrations of the Tetracycline-laden shell. The study's findings indicate that at a concentration of 300 ppm, optimal antibacterial efficacy was achieved, with an inhibition zone diameter of 22.5 ± 0.1 mm and a MIC of 25 μg/mL. It can be concluded that the CTS-Brij S100 system holds potential for applications in facilitating the delivery of the antibiotic Tetracycline.