| dc.description.abstract | Hydrogen sulfide (H₂S) plays a crucial role in regulating various disease processes, such
as safeguarding mammalian cells from harm, stimulating tissue regeneration, and
managing illnesses stemming from physiological imbalances. However, existing methods
faced challenges due to the unpredictable release of H₂S. This research introduced an H₂S
delivery system by enclosing 5-aminopyridine-2-thiocarboxamide (APTC) within a
gelatin-pluronic (GP) thermosensitive hydrogel. This approach enabled a controlled and
gradual release of H₂S, leveraging the thermosensitive properties of the GP copolymer
that allowed for in situ formation at body temperature. This system showed promise as an
injectable biomaterial for medical purposes. The study proved that the GP-based hydrogel
effectively controlled the release of H₂S. There was a direct relationship between the
concentration of APTC during production and the amount of H₂S released, where higher
APTC levels led to increased H₂S release, as evidenced by the Methylene Blue assay. The
controllable and sustained release of H₂S offered significant advantages, including
enhanced proliferation of endothelial cells such as human dermal fibroblasts (hDFBs), as
demonstrated by the WST-1 assay. Overall, these results highlighted the potential of
pluronic-based hydrogels as a reliable H₂S delivery method, paving the way for designing
tailored thermosensitive hydrogels for potential clinical use. | en_US |
