| dc.description.abstract | The study investigates the efficiency of the extract obtained from Houttuynia Cordata Thunb leaves
under different conditions and evaluates its phytochemical, antioxidant, and antimicrobial properties
using Sonication for Serum Formulation. The results indicate that ethanol solvent, coupled with
sonication and a rotary evaporator at various temperatures (30°C, 50°C, and 70°C), yields the most
efficient extraction at 50°C. Total phenolic contents at these temperatures range from 13.50 ± 0.015
to 39.99 ± 0.030 mg GAE/g extract, with the highest level observed at 70°C. Conversely, total
flavonoid contents are lower than total phenolic contents, ranging from 10.92 ± 0.015 to 17.25 ± 0.010
mg QE/g extract at 30°C, 50°C, and 70°C, respectively. The extract demonstrates moderate
antioxidant activity, with an IC50 value of 45.43 mg/ml, compared to the control sample of Ascorbic
Acid (IC50 = 3.2 mg/ml) under various experimental concentrations. Additionally, it exhibits moderate
resistance against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa under
experimental conditions. High-performance liquid chromatography (HPLC) analysis reveals the
presence of Rutin, Quercetin, and Chlorogenic Acid compounds, while Kaempferol and Vitexin are
not detected in the Houttuynia Cordata extract. In the antimicrobial assay, inhibition zones are
observed at 10 ± 0.2 mm for Escherichia coli, 6.0 ± 0.0 mm for Staphylococcus aureus, and 13.0 ±
0.2 mm for Pseudomonas aeruginosa. The Minimum Inhibitory Concentration (MIC) concentrations
vary, ranging from 0.5 μg/ml to 12.5 mg/ml, with the lowest MIC value of 7.5 observed for the sample
at 70°C against Escherichia coli bacteria.
The pH of the preparation is tested and found to be approximately 6.69 to 7.0, suitable for skin
application. Overall, the total phenolic and flavonoid contents, along with the high antioxidant
capacity of the extract, provide supportive evidence for further research on the potential application
of Houttuynia Cordata in cosmetic products. | en_US |
