| dc.description.abstract | In this study, project mainly focused on investigating the development of cloned
bovine embryos in preimplantation as well as the expression of histone acetylation
during the first cell cycle after changing the location of somatic cell nucleus which
was injected into enucleated mature bovine oocytes (Somatic Cell Nuclear Transfer,
SCNT). In each group, the control groups were used for transferring cell nuclear in
the enucleated area (the location of metaphase II), while the experiment groups were
designed for injecting in different locations, such as opposite (1800 degree) and
square (900 degree) angle sites to the location of metaphase II. The research was
designed for 2 experiments, which are to evaluate the preimplantation development
of cloned bovine embryos in different positions of somatic cell nucleus injection into
enucleated oocytes; and analyze histone acetylation expressed in the first cell cycle
(10 hours of post-activation) of those location. In the first experiment, the
preimplantation development was only approximately 23% in blastocyst formation.
Besides, there were no significant differences from 2 cell stage to blastocyst stage in
development and survival rate (p>0,05) between each group. In the second
experiment, the location of fibroblast cell injection at 90o direction and 180o direction
did not impact the expression of histone H3 lysine 9 acetylation (acH3K9) based on
the intensity in each group (p>0,05). Moreover, all of those still form the pronuclei
in the first cell cycle, leading to the similarity in all groups of the experiment. In
conclusion, changing the location injection during SCNT does not have any impact on
the development of the SCNT bovine embryo during preimplantation as well as the
H3K9Ace modification | en_US |
