| dc.description.abstract | Zoonotic diseases, those transmitted from animals to humans, pose significant public health
threats globally. Understanding and monitoring these diseases, such as Staphylococcus
aureus infections, particularly in domesticated environments like wild boars, is crucial due
to their potential to serve as reservoirs or vectors of transmission to humans. This study
aimed to evaluate the presence of Staphylococcus aureus on domesticated wild boars and
farm workers, particularly in nasal cavities. In this study, we employed a comprehensive
approach involving morphological examination, biochemical characterization via
coagulase testing, PCR for detecting the spa gene, 16S rRNA targeting identification, and
pairwise distance calculation to analyze 74 Staphylococcus-like isolates. The aim was to
assess the prevalence of Staphylococcus aureus in two distinct hosts: humans and wild
boars. Our findings revealed that out of the 74 isolates studied, only 2 were identified as
Staphylococcus aureus from human samples, with a calculated pairwise distance of
0.00172. Notably, no evidence of S. aureus presence was detected in the nasal cavities of
wild boars, indicating a 0% prevalence in this host population. The observed differences in
S. aureus presence between humans and wild boars underscore the need for further
investigation. Additional sampling across broader geographical areas and larger sample
sizes are essential to verify and generalize these findings. Moreover, enhancing
identification techniques, possibly through advanced genomic methods or metagenomic
analyses, could provide more precise insights into the distribution and dynamics of S.
aureus across different host species. In conclusion, while our initial results provide
valuable insights, they highlight the necessity for continued research efforts to confirm
these prevalence percentages and explore potential ecological factors influencing S. aureus
colonization in diverse host environments. This approach will contribute to a more
comprehensive understanding of microbial ecology and host-pathogen interactions, with
implications for public health and wildlife management strategies. | en_US |
