| dc.description.abstract | As a pioneer in tropical pasture legumes, Stylosanthes guianensis, commonly known as
stylo, exhibits excellent adaptation to growth-limiting factors in acidic soils. Considering
the importance of stylo, there is a need to induce hairy roots and leverage their specific
characteristics for further exploration of ecological and symbiotic root systems. This study
employed Stylosanthes guianensis cv. HAMATA to systematically optimize
Agrobacterium-mediated transformation for inducing the hairy root phenotype. The
results demonstrated that surface sterilization of seeds using a 3% concentration of
hydrogen peroxide disinfectant solution was optimal for in vitro seedlings, resulting in the
highest germination rate and lowest contamination levels. The highest rate of root
induction was achieved with a 15-minute infection time and 3 days of co-cultivation.
Subsequent polymerase chain reaction (PCR) analysis confirmed successful integration of
the rolB gene into the stylo genome. While this protocol enhances hairy root induction,
wider testing is warranted | en_US |
