| dc.description.abstract | Foodborne illness is a medical disorder that arises after consuming contaminated food
products containing dangerous bacteria, viruses, or parasites. Bacteria present in meat
products that can cause foodborne disease include Staphylococcus aureus (S. aureus),
Klebsiella spp., Vibrio spp. This study aims to detect the prevalence of the
aforementioned microbes and check their antibiotic susceptibility. A total of 18 meat
samples including 6 pork samples, 6 chicken samples, 6 beef samples were purchased at
3 different open markets from September 2023 to January 2024. Isolation of target
microbes was based on QCVN 01-04:2009/BNNPTNT guidelines, and the target
microbes was characterized via Gram-staining and their corresponding biochemical tests.
After being characterized, the antibiotic susceptibility test was conducted by using agar
well diffusion method. The isolates were tested with 5 antibiotics namely ampicillin,
cefotaxime, ciprofloxacin, gentamicin, streptomycin. The resistance and susceptibility
breakpoints were recorded following the MIC guidelines of the Clinical and Laboratory
Standards Institute (CLSI) and European Committee on Antimicrobial Susceptibility
Testing (EUCAST). Consequently, a total of 166 isolates were characterized from 18
meat samples, 166 isolates consisted of 131 (79%) S. aureus, 10 (6%) Klebsiella spp.,
25 (15%) Vibrio spp. isolates. There were 100 isolates (79 S. aureus, 7 Klebsiella spp.,
14 Vibrio spp. isolates) were tested to determine the antibiotic susceptibility. All 3 target
microbes shown resistance to streptomycin with over 90%, to ampicillin with more than
50%, to gentamicin with over 40%. The results indicated a high prevalence of microbes
and high resistance rate. However, there were still some limitations and challenges in this
study that can be improved for future projects. | en_US |
