| dc.description.abstract | Interspecies Somatic Cell Nuclear Transfer (iSCNT) played an important role in aspect of
cloning animals in the Biotechnology industry. Especially, the mechanism of
reprogramming of iSCNT was still not clear which led to the failure of iSCNT. In this
experiment, we performed experiments involving iSCNT in two species, Bovine and
Mouse, to investigate remodeling of chromosome after iSCNT and pronuclear formation
of iSCNT cloned embryos during the first mitotic division. Here, we conducted two
experiments specifically targeting the Bovine oocyte and Mouse oocyte groups, with the
donor cell being fibroblasts established from both Bovine and Mouse. These groups were
further subdivided into two subgroups: the control group (consisting of the same species
of oocyte and donor cell) and the iSCNT group (with different species between the oocyte
and donor cell). This total includes the Mouse control, Mouse iSCNT, Bovine control, and
Bovine iSCNT groups. After completing the experiments, we collected results from the
two experimental groups of Mouse oocytes and Bovine oocytes. We measured the
intensity from images of acetylation expression to gather data regarding the formation of
pronuclei. For the Mouse oocyte group, the intensity level of H3K9Ace of the mouse
control was higher than iSCNT group (16.26 ≥ 15.77, respectively, p<0.05) at pronuclear
formation phage. Similarly, for the Bovine oocyte group, the level of H3K9Ace of bovine
control was lower than bovine iSCNT group (24.96 ≤ 78.01, respectively, p<0.05) The
results demonstrated that the nuclei still had potential remodel despite of nuclei and
enucleated oocyte coming from different strains. | en_US |
