| dc.description.abstract | Embryonic stem (ES) cells are a model with great potential in regenerative medicine and
study of developmental biology. The knowledge gained in mice can be applied to humans
to develop potential methods for many different traits. In this study, ESC-like clusters
were obtained from blastomeres biopsied from 2- and 8-cell ICR mouse embryos using
the preimplantation genetic diagnosis (PGD) like technique and then cultured in stem cell
(SC) culture medium. Afterward, the biopsied blastomeres were cultured in SC medium
and observed for morphology development as well as the duration required for ES cluster
formation. The studies indicated that over 7 days of culture duration in SC medium, both
2-cell blastomeres and 8-cell blastomeres had the ability to grow into ES clusters.
However, it is highlighted that the formation rate of ES cluster derived from 2-cell isolated
blastomeres at 13.33% was lower than the one from 8-cell blastomeres at 26.13%. Due
to the outcomes, the recovery capacity in the SC medium of 8-cell blastomeres might have
been higher than those from 2-cell embryos. | en_US |
