Assessing characteristics of the fibrin gel from bovine plasma

Abstract

Fibrin is regarded as a very promising biomaterial with a wide range of therapeutic uses. Despite being widely recognized in the field of tissue engineering, the field of in vitro meat has been recently increasing. Agriculture provides a good source of meat for humans, however, the demand for meats increases followed by an increase in global population. In this study, the plasma was collected from bovine blood, which would be used as a source to make a fibrin gel. Fibrinogens and other important proteins in plasma create clotting material for wounds, so the scaffold made from plasma is biomaterial. The concentration of protein components in plasma was assessed by Ethanol absolute since there was plenty of protein in the plasma, after assessing the total protein components, the concentration of a specific protein which was fibrinogen was assessed. Depending on the value of this assessment, it was able to reserve the plasma maintaining the concentration of fibrinogen for long-term experiments. This reserved plasma was then formed into a fibrin gel by the addition of calcium ions. The fibrin gel is made from the overlap of fibrin fibers creating a porous structure that allows the cells to attach to. The Hematoxylin and Eosin stain was used to detect the fibrin fibers which were an acidophile and would be attached by Eosin and would demonstrate the pink to dark pink color. However, the H&E stain was only able to observe the fibrin gel in 2 dimensions, the Scanning Electron Microscope was used to observe the fibrin in 3 dimensions to clarify the porous structure and fibrin fibers. This fibrin gel was also assessed to be clotted as fast as possible because the mixture of cell and culture medium was added to the mixture of plasma and calcium ion, then the fibrin gel would clot and encapsulate the cells inside