| dc.description.abstract | African swine fever (ASF) is a highly contagious viral illness that mostly affects
farmed pigs and wild boars. This infection is caused by the African swine fever
virus (ASFV), which belongs to the Asfarviridae family. ASF is characterized by a
variety of clinical signs, including high fever, hemorrhage, and high mortality in
affected pigs. Infection is mainly transmitted through contact with contaminated
animals, ingestion of contaminated food or infectious objects, or by ticks of the
class Ornithodoros.
PCR, or polymerase chain reaction, is a widely utilized technology in the field of
diagnostics. It is commonly employed by researchers to diagnose illnesses, clone,
and sequence genes, and conduct intricate genetic and quantitative investigations
in a rapid manner and quite responsive. (Garibyan and Avashia in 2013). TA
cloning is a significant system that utilizes the unpaired A overhang present at the
3' end of PCR products generated with unproofreaded DNA polymerase. (Carson
et al., 2019). This work has successfully generated recombinant DNA by employing
the aforementioned approaches. The recombinant DNA includes the ASF p30 gene
and a cloning vector, which serves as a positive control for molecular biology
experiments.
ASF has a substantial economic effect on the swine business, requiring new
regulations, quarantining infected organisms, and interrupting pork production.
Despite efforts to control its spread, ASF remains a concern worldwide due to its
high mortality rate, need for antibodies, and challenges in eradication. Feasibility
monitoring, biosecurity measures developing rapid, accurate diagnostics, and
enhancing quality control are essential to manage and prevent the spread of ASF. | en_US |
