| dc.description.abstract | In-situ formed hydrogel is a type of biomaterial that is attractively utilized for biomedical
applications. In recent decades, compounds like hydrogel have gained significant research
attention due to their versatile applications. These materials find use in various fields,
particularly in the realm of biomedical engineering. Crosslinking through enzyme-catalyzed
reactions often exhibits high specificity, and excellent biocompatibility, and avoids adverse
reactions during the crosslinking process in the field of biomedicine. Among these,
horseradish peroxidase (HRP) emerges as a potential candidate for hydrogel formation, but
certain limitations have impacted the practicality of the product, such as HRP becoming
inactive at high H2O2 concentrations. In this context, we prepared an alginate-tyramine histidine-hematin (Alg-Ta-His-He) polymer which could be exploited to prepare an
injectable hydrogel in the absence of Horseradish peroxidase (HRP). The chemical structure
of Alg-Ta-His-He was characterized by UV-Vis, and FTIR spectroscopies. The Alg-Ta-His He hydrogel could be formed in situ rapidly in the presence of a certain amount of H2O2
concentration. Besides, Alg-Ta-His-He can catalyze pyrogallol and guaiacol like the enzyme
HRP, and Alg-Ta-His-He is highly compatible with cells, with 100% viability of human
fibroblast cells in vitro. In summary, this method opens up the opportunity to effectively use
hematin as an effective alternative to HRP enzyme in hydrogel formation, reducing costs
and increasing applicability in biomedical and engineering applications. | en_US |
