| dc.description.abstract | Tuberculosis (TB) is the world's 13th leading cause of mortality and the
second most transmissible cause of death after COVID-19. Despite the existence
of vaccines and therapies, TB remains a significant threat if it is not properly
recognized. It affects the respiratory system, causing chronic coughs, chest pain,
and coughing up blood or mucus. TB can be categorized as latent TB infection
(LTBI) or TB disease, with untreated TB potentially resulting in mortality.
Presently, Tuberculosis bacteria are identified by a variety of techniques,
including sputum testing using Ziehl stain, culture, X-ray imaging, Mantoux
response, or bronchoscopy. Nevertheless, these technologies are not without their
limits, including concerns about time and accuracy. The culture method is often
regarded as the most reliable and accurate; however, the slow division of
Mycobacterium tuberculosis leads to unpredictable findings. Only a small number
of level 3 laboratories in Vietnam are authorized to transport tuberculosis bacterial
samples solely for research purposes. However, ethical considerations also arise
from regularly gathering samples from patients with tuberculosis. PCR, a
significant breakthrough in molecular biology, is utilized in biomedical diagnosis to
address the aforementioned issues. PCR is highly sensitive, but it is susceptible to
many factors that can lead to false positive or false negative results, impacting
the accuracy of the diagnosis. Thus, there is a necessity for an approach to provide
quality control. TA cloning is another crucial technique that enables the generation
of positive controls by inserting DNA segments into a plasmid vector. The IS6110
gene is widely present and frequently used to identify and distinguish Mycoplasma
tuberculosis DNA in clinical samples using PCR. Hence, the aim of this research is
to enhance the detection of tuberculosis by employing TA cloning methods to
construct a plasmid that harbors the IS6110 gene. It will act as a positive control
sample, ensuring the quality control of the test and reducing the need for multiple
samples. The project aims to lay the foundation in the laboratory for future
research focused on improving the sensitivity of TB detection methods. According
to the study's findings, the researchers can accurately and efficiently detect
tuberculosis with a high level of reliability. Moreover, this study is expected to
increase the ability to proactively generate more positive controls for other
pathogens in the future. | en_US |
