Optimizing the PCR process to identify Candida spp. species

Abstract

Fungi that can cause disease include ringworm and yeast. Currently, Candida fungus is gradually becoming popular in Vietnam. It often causes diseases of the skin and mucous membranes in the form of thrush, vulvovaginitis, paronychia, etc. There are many commonly developed methods for distinguishing Candida spp. Such as: Germ tube test or Kit Rapid Yeast Plus or Chromogen Candida, ... However, these methods can take a long time to get results or sometimes there will be errors leading to incorrect treatment indications, Quickly and promptly. For the PCR method, the advantage is that it gives fast and highly accurate results, helping to improve the efficiency of medical examination and treatment for patients. This paper discusses the development and optimization of a procedure for the detection of Candida spp. Using Direct PCR method to shorten species identification time while bringing high accuracy and saving time for patients. Compare results between methods to evaluate the effectiveness of this optimization process. Then, sequenced to examine specificity.