| dc.description.abstract | Hair follicle stem cells (HFSCs) represent a promising and readily accessible source
of mesenchymal stem cells (MSCs), known for their multipotent characteristics and
non-oncogenic features, which make them ideal candidates for various regenerative
medicine applications. Cryopreservation is a critical technique that enables the long term storage of these cells, preserving their viability for future research and therapeutic
use. This study focused on evaluating the viability, morphology, and growth
characteristics of cryopreserved bovine hair follicle-derived cells (bHFCs) during in
vitro expansion following the thawing process. After analysis of multiple passages,
revealed significant morphological changes, including cytoplasmic and nuclear
enlargement, particularly in later passages, which are indicative of cellular senescence.
The study also observed an increase in apoptosis rates and a corresponding decrease
in cell viability as the passages progressed. Flow cytometry analysis further indicated
a G2/M phase arrest in the later passages, suggesting a disruption in the normal cell
cycle progression. Additionally, Realtime qRT-PCR analysis demonstrated strong
expression of key MSC markers, such as CD44, CD73, CD90, and CD105 with a
significant increase in expression at later passage. These molecular markers confirmed
the preservation of essential MSC characteristics in the cryopreserved bHFCs, despite
the onset of senescence and other changes. The results of this study highlight the
potential of cryopreserved bHFCs as a viable and valuable resource for regenerative
medicine, offering a stable and multipotent cell source for future therapeutic
applications. This underscores the importance of cryopreservation in maintaining the
functionality of HFSCs over extended periods, ensuring their continued relevance in
stem cell research and future applications. | en_US |
